Lei Yuan, Licheng Zhang, Xiao Wei, Ruihua Wang, Nannan Li, Gaili Chen, Fengfeng Fan, Shaoying Huang, Jianxiong Li, Shaoqing Li

Int J Mol Sci.; 2022 Feb 21;23(4):2379.  doi: 10.3390/ijms23042379.

Abstract

Salt stress is one of the most severe adverse environments in rice production; increasing salinization is seriously endangering rice production around the world. In this study, a rice backcross inbred line (BIL) population derived from the cross of 9311 and wild rice Oryza longistaminata was employed to identify the favorable genetic loci of O. longistaminata for salt tolerance. A total of 27 quantitative trait loci (QTLs) related to salt tolerance were identified in 140 rice BILs, and 17 QTLs formed seven QTL clusters on different chromosomes, of which 18 QTLs were derived from O. longistaminata, and a QTL for salt injury score (SIS), water content of seedlings (WCS) under salt treatment, and relative water content of seedlings (RWCS) was repeatedly detected and colocalized at the same site on chromosome 2, and a cytochrome P450 86B1 (MH02t0466900) was suggested as the potential candidate gene responsible for the salt tolerance based on sequence and expression analysis. These findings laid the foundation for further improving rice salt tolerance through molecular breeding in the future.

See: https://pubmed.ncbi.nlm.nih.gov/35216499/

Fig.3: QTLs identified in the O. longistaminata for salt tolerance. Left is the scale for the genetic length of each chromosome. The upward direction indicates the locus comes from O. longistaminata, while the downward direction indicates the locus comes from 9311. The blue indicates QTLs detected in the first experiment and the red indicates QTLs detected in the second experiment. cM, centi-Morgan.