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| Research Pinpoints Key Gene in Pollen Development |
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Maize gene ZmADF1 negatively regulates pollen vitality, according to Zhejiang Academy of Agricultural Sciences researchers and partners. Their findings are reported on Biochemical and Biophysical Research Communications. Normal pollen grain development and achievement of double fertilization are vital in the sexual reproduction of angiosperms and the production of grains. Actin depolymerizing factor (ADF) controls the growth, development, and responses to environmental stress and diseases by binding to actin in plants. |
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Maize gene ZmADF1 negatively regulates pollen vitality, according to Zhejiang Academy of Agricultural Sciences researchers and partners. Their findings are reported on Biochemical and Biophysical Research Communications.
Normal pollen grain development and achievement of double fertilization are vital in the sexual reproduction of angiosperms and the production of grains. Actin depolymerizing factor (ADF) controls the growth, development, and responses to environmental stress and diseases by binding to actin in plants. Thus, the researchers investigated the role of ZmADF1 in pollen development and other processes through bioinformatic analysis, subcellular localization, overexpression, and knockout using CRISPR-Cas9.
Results showed that ZmADF1 is specifically expressed in maize pollen and highly similar to ADFs of other species. When ZmADF1 was overexpressed, a significantly reduced pollen germination rate was observed in Arabidopsis, while the rate of empty shells in fruit pods increased. In maize, ZmADF1 negatively regulated pollen vitality through overexpression and knocking out of ZmADF1.
Read the open-access article for more information.
See https://www.isaaa.org/kc/cropbiotechupdate/ged/article/default.asp?ID=20734 |
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