Mian Wang, Lifei Zhu, Chushu Zhang, Haixiang Zhou, Yueyi Tang, Shining Cao, Jing Chen, Jiancheng Zhang

Int J Mol Sci.; 2024 Jan 3; 25(1):619. doi: 10.3390/ijms25010619.

Abstract

Peanut Fusarium rot, which is widely observed in the main peanut-producing areas in China, has become a significant factor that has limited the yield and quality in recent years. It is highly urgent and significant to clarify the regulatory mechanism of peanuts in response to Fusarium oxysporum. In this study, transcriptome and proteome profiling were combined to provide new insights into the molecular mechanisms of peanut stems after F. oxysporums infection. A total of 3746 differentially expressed genes (DEGs) and 305 differentially expressed proteins (DEPs) were screened. The upregulated DEGs and DEPs were primarily enriched in flavonoid biosynthesis, circadian rhythm-plant, and plant-pathogen interaction pathways. Then, qRT-PCR analysis revealed that the expression levels of phenylalanine ammonia-lyase (PAL), chalcone isomerase (CHI), and cinnamic acid-4-hydroxylase (C4H) genes increased after F. oxysporums infection. Moreover, the expressions of these genes varied in different peanut tissues. All the results revealed that many metabolic pathways in peanut were activated by improving key gene expressions and the contents of key enzymes, which play critical roles in preventing fungi infection. Importantly, this research provides the foundation of biological and chemical analysis for peanut disease resistance mechanisms.

See https://pubmed.ncbi.nlm.nih.gov/38203792/

Figure 5:

A KEGG enrichment analysis of DEPs. The vertical axis is protein-enriched KEGG pathway and the horizontal axis is protein p-value in −log 10. (A) The pathway of upregulated DEPs enrichment. (B) The pathway of downregulated DEPs enrichment.