Home >> News >> Publications >> PhD.Thesis >> Vu Quoc Luan. 2016. Optimizing the regeneration frequency in micropropagation of Paphiopedium sp. PhD Thesis. The Institute of Agricultural Sciences for Southern Vietnam. Ho Chi Minh City, VIETNAM
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- - What do you think of this newsletter?
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Achievement
Independence Award
- First Rank - Second Rank - Third Rank
Labour Award
- First Rank - Second Rank -Third Rank
National Award
- Study on food stuff for animal(2005)
- Study on rice breeding for export and domestic consumption(2005)
VIFOTEC Award
- Hybrid Maize by Single Cross V2002 (2003)
- Tomato Grafting to Manage Ralstonia Disease(2005)
- Cassava variety KM140(2010)
Centres
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Department of Biotechnology
https://sites.google.com/site/cadcnshias/ -
Dalat center
http://pvfcdalat.vn -
Hung Loc Agricultural Research Center
http://harc-ias.vn/
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| Vu Quoc Luan. 2016. Optimizing the regeneration frequency in micropropagation of Paphiopedium sp. PhD Thesis. The Institute of Agricultural Sciences for Southern Vietnam. Ho Chi Minh City, VIETNAM | ||||||||||
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The percentage of regenerants by Paphiopedium landraces viz. Van Hai, Lan Hai Hong and Lan Tam Dao obtained 22.40, 24.85 and 17.15 %, respectively under the SH medium plus 1.0 mg/L of TDZ, 0.3 mg/L of NAA, 9 g/L of agar and 30 g/L of sucrose at pH of 5.8 |
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[submitted in Jan 17, 2017 at the IAS] ABSTRACTThe percentage of regenerants by Paphiopedium landraces viz. Van Hai, Lan Hai Hong and Lan Tam Dao obtained 22.40, 24.85 and 17.15 %, respectively under the SH medium plus 1.0 mg/L of TDZ, 0.3 mg/L of NAA, 9 g/L of agar and 30 g/L of sucrose at pH of 5.8 Under condition of green LED (100%) plus suitable medium of liquid SH medium + 1 mg/L of TDZ, 0.3 mg/L of NAA, 30 g/L of sucrose, pH = 5.8; the regenerated plants of Van Hai, Lan Hai Hong and Lan Tam Dao obtained 48.45, 44.90 and 25.30 %, respectively. The callus induction from shoot nodes by Van Hai offered the highest percentage of 31.25%. The wound induction to create lateral buds successfully expressed better micropropation among three Paphiopedium landraces. Three created protocols that could optimize the micropropagation of Paphiopedium were proposed to be applied. |
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