Welcome To Website IAS

Hot news
Achievement

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)

VIFOTEC Award

- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Centres
Website links
Vietnamese calendar
Library
Visitors summary
 Curently online :  3
 Total visitors :  5123118

A Raf-like kinase is required for smoke-induced seed dormancy in Arabidopsis thaliana
Friday, 2021/04/09 | 20:46:37

Inhye Lee, Eunsun Kim, Soobin Choi, Dayoung Kim, Wangyu Hong, Jungki Choi, Hyunmo Choi, Jimin Kim, Ganesh A. Sable, Kesavan Markkandan, Dongyeol Lim, Soon Ki Park, Soo Young Kim, Sumin Lee, and Moon-Soo Soh

 

PNAS April 6, 2021 118 (14) e2020636118

Significance

Seed dormancy is an important developmental trait for plants, which ensures seed germination under more favorable spatiotemporal environments. A smoke compound, 3,4,5-trimethylfuran-2(5H)-one, which is called TMB for short, has been identified to inhibit seed germination in a still elusive way. Here, we presented several lines of evidence that TMB inhibits seed germination through the induction of physiological dormancy. Furthermore, by isolating TMB-resistant Arabidopsis mutants, we demonstrated that TMB RESISTANT1, encoding a Raf-like kinase, is critically required for the TMB-induced seed dormancy. These results suggest the presence of a smoke sensory pathway that takes part in the multiple layers of the dormancy regulatory network in higher plants.

Abstract

Plants sense and integrate diverse stimuli to determine the timing for germination. A smoke compound, 3,4,5-trimethylfuran-2(5H)-one (trimethylbutenolide, TMB), has been identified to inhibit the seed germination of higher plants. To understand the mode of action, we examined various physiological and molecular aspects of the TMB-dependent inhibition of seed germination in Arabidopsis thaliana. The results indicated that the effect of TMB is due to the enhanced physiological dormancy, which is modulated by other dormancy regulatory cues such as after-ripening, stratification, and ABA/GA signaling. In addition, gene expression profiling showed that TMB caused genome-wide transcriptional changes, altering the expression of a series of dormancy-related genes. Based on the TMB-responsive physiological contexts in Arabidopsis, we performed mutant screening to isolate genetic components that underpin the TMB-induced seed dormancy. As a result, the TMB-RESISTANT1 (TES1) gene in Arabidopsis, encoding a B2 group Raf-like kinase, was identified. Phenotypic analysis of the tes1 mutant implicated that TES1 has a critical role in the TMB-responsive gene expression and the inhibition of seed germination. Taken together, we propose that plants have been equipped with a TMB sensory pathway through which the TMB induces the seed dormancy in a TES1-dependent way.

 

See https://www.pnas.org/content/118/14/e2020636118

 

Figure 1:

The TMB inhibits the seed germination of Arabidopsis. (A) Chemical structures of KAR1 and TMB. (B) The effect of the after-ripening on the TMB-dependent inhibition of germination. The dry-stored wild-type (Col-0) seeds for the indicated time (weeks) after harvest were sown on the aqueous media with or without 50 μM TMB. The germination was counted based on the cotyledon emergence at 5 d after incubation under continuous white light. Mean values (± SD) of germination rates were determined from three experimental replicates with at least 50 seeds per plate. (C) The TMB-dependent inhibition of the germination of freshly matured Arabidopsis seeds. The freshly matured wild-type (Col-0) seeds were imbibed on the aqueous media either containing mock or 50 μM TMB. The germination was counted based on the cotyledon emergence. Mean values (± SD) of germination rates were determined from three experimental replicates with at least 50 seeds per plate. (D) The dose response of the TMB-dependent inhibition of the germination. The freshly matured wild-type (Col-0) seeds were sterilized and imbibed on the aqueous media containing various concentrations of TMB as indicated. The germination rate was counted based on the cotyledon emergence at 4 d after sowing. Mean values (± SD) of germination rates were determined from three experimental replicates with at least 50 seeds per plate. (E) The effect of stratification on the germination inhibitory effect of the TMB. The sterilized freshly matured wild-type (Col-0) seeds were water imbibed and kept at 4 °C for the indicated days, and then the seeds were sown on the aqueous media either with or without 50 μM TMB. The germination was counted based on the cotyledon emergence at 5 d after sowing. Mean values (± SD) of germination rates were determined from three experimental replicates with at least 50 seeds per plate. (F) The KAI2-independent action of the TMB on the inhibition of the germination. The freshly matured wild-type Landsberg erecta (Ler) and kai2-2 seeds were sterilized and imbibed on the aqueous media without (Mock) or with 50 μM TMB (TMB), 10 μM KAR1 (KAR), or both 50 μM TMB and 10 μM KAR1 (TMB+KAR) under the continuous light condition. The germination was counted based on the cotyledon emergence at 4 d after sowing. Mean values (± SD) of germination rates were determined from three experimental replicates with at least 50 seeds per plate.

Back      Print      View: 22

[ Other News ]___________________________________________________
  • Genome-wide analysis of autophagy-associated genes in foxtail millet (Setaria italica L.) and characterization of the function of SiATG8a in conferring tolerance to nitrogen starvation in rice.
  • Arabidopsis small nucleolar RNA monitors the efficient pre-rRNA processing during ribosome biogenesis
  • XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves.
  • Reducing the Use of Pesticides with Site-Specific Application: The Chemical Control of Rhizoctonia solani as a Case of Study for the Management of Soil-Borne Diseases
  • OsJRL, a rice jacalin-related mannose-binding lectin gene, enhances Escherichia coli viability under high-salinity stress and improves salinity tolerance of rice.
  • Production of lipopeptide biosurfactants by Bacillus atrophaeus 5-2a and their potential use in microbial enhanced oil recovery.
  • GhABF2, a bZIP transcription factor, confers drought and salinity tolerance in cotton (Gossypium hirsutum L.).
  • Resilience of cassava (Manihot esculenta Crantz) to salinity: implications for food security in low-lying regions.
  • Cellulose synthase complexes act in a concerted fashion to synthesize highly aggregated cellulose in secondary cell walls of plants
  • No adverse effects of transgenic maize on population dynamics of endophytic Bacillus subtilis strain B916-gfp
  • Identification and expression analysis of OsLPR family revealed the potential roles of OsLPR3 and 5 in maintaining phosphate homeostasis in rice
  • Functional analysis of molecular interactions in synthetic auxin response circuits
  • Titanium dioxide nanoparticles strongly impact soil microbial function by affecting archaeal nitrifiers.
  • Inducible Expression of the De-Novo Designed Antimicrobial Peptide SP1-1 in Tomato Confers Resistance to Xanthomonas campestris pv. vesicatoria.
  • Toward combined delignification and saccharification of wheat straw by a laccase-containing designer cellulosome
  • SNP-based discovery of salinity-tolerant QTLs in a bi-parental population of rice (Oryza sativa)
  • Pinpointing genes underlying the quantitative trait loci for root-knot nematode resistance in palaeopolyploid soybean by whole genome resequencing.
  • Transcriptome- Assisted Label-Free Quantitative Proteomics Analysis Reveals Novel Insights into Piper nigrum -Phytophthora capsici Phytopathosystem.
  • Brassinosteroids participate in the control of basal and acquired freezing tolerance of plants
  • Rapid hyperosmotic-induced Ca2+ responses in Arabidopsis thaliana exhibit sensory potentiation and involvement of plastidial KEA transporters

 

Designed & Powered by WEBSO CO.,LTD