Nayak SN, Agarwal G, Pandey MK, Sudini HK, Jayale AS, Purohit S, Desai A, Wan L, Guo B, Liao B, Varshney RK.
Sci Rep. 2017 Aug 29;7(1):9659. doi: 10.1038/s41598-017-09260-8.
Abstract
Aflatoxin contamination, caused by fungal pathogen Aspergillus flavus, is a major quality and health problem delimiting the trade and consumption of groundnut (Arachis hypogaea L.) worldwide. RNA-seq approach was deployed to understand the host-pathogen interaction by identifying differentially expressed genes (DEGs) for resistance to in-vitro seed colonization (IVSC) at four critical stages after inoculation in J 11 (resistant) and JL 24 (susceptible) genotypes of groundnut. About 1,344.04 million sequencing reads have been generated from sixteen libraries representing four stages in control and infected conditions. About 64% and 67% of quality filtered reads (1,148.09 million) were mapped onto A (A. duranensis) and B (A. ipaёnsis) subgenomes of groundnut respectively. About 101 million unaligned reads each from J 11 and JL 24 were used to map onto A. flavus genome. As a result, 4,445 DEGs including defense-related genes like senescence-associated proteins, resveratrol synthase, 9s-lipoxygenase, pathogenesis-related proteins were identified. In A. flavus, about 578 DEGs coding for growth and development of fungus, aflatoxin biosynthesis, binding, transport, and signaling were identified in compatible interaction. Besides identifying candidate genes for IVSC resistance in groundnut, the study identified the genes involved in host-pathogen cross-talks and markers that can be used in breeding resistant varieties.
See: https://www.ncbi.nlm.nih.gov/pubmed/28851929
Figure 1: Phenotypic observations for seeds of J 11 and JL 24 during in-vitro seed colonization by Aspergillus flavus at different time points along with microscopic observation and aflatoxin estimation. The figure shows the microscopic observations of seed coat after fungal inoculation in J 11 (resistant) and JL 24 (susceptible) that clearly shows the presence of mycelium at Day 3 and sporulation at Day 7 after inoculation.
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