Sun Y, Mu C, Chen Y, Kong X, Xu Y, Zheng H, Zhang H, Wang Q, Xue Y, Li Z, Ding Z, Liu X.
Plant Physiol Biochem. 2016 Oct 22;109:467-481. doi: 10.1016/j.plaphy.2016.10.017. [Epub ahead of print]
Abstract
Maize (Zea mays L.) is an important food and energy crop, and low phosphate (Pi) availability is one of the major constraints in maize production worldwide. Plants adapt suitably to acclimate to low Pi stress. However, the underlying molecular mechanism of Pi deficiency response is still unclear. In this study, comparative transcriptomic analyses were conducted to investigate the differences of transcriptional responses in two maize genotypes with different tolerances to low phosphorus (LP) stress. LP-tolerant genotype QXN233 maintained higher P and Pi levels in shoots than LP-sensitive genotype QXH0121 suffering from Pi deficiency at seedling stage. Moreover, the transcriptomic analysis identified a total of 1391 Pi-responsive genes differentially expressed between QXN233 and QXH0121 under LP stress. Among these genes, 468 (321 up- and 147 down-regulated) were identified in leaves, and 923 (626 up- and 297 down-regulated) were identified in roots. These Pi-responsive genes were involved in various metabolic pathways, the biosynthesis of secondary metabolites, ion transport, phytohormone regulation, and other adverse stress responses. Consistent with the differential tolerance to LP stress, five maize inorganic Pi transporter genes were more highly up-regulated in QXN233 than in QXH0121. Results provide important information to further study the changes in global gene expression between LP-tolerant and LP-sensitive maize genotypes and to understand the molecular mechanisms underlying maize's long-term response to Pi deficiency.
See: https://www.ncbi.nlm.nih.gov/pubmed/27825075
Fig. 8.
Overview of differentially expressed genes (DEGs) between QXH0121 and QXN233 under the normal and LP conditions. A total of 7910 and 1391 genes in the control and LP-treated groups, respectively, were identified to be expressed differentially between QXH0121 and QXN233 plants according to our thresholds fold change of at least ± 2 and FDR ≤ 0.001. (A) Number of DEGs in leaves and roots of QXN233 compared with QXH0121 at the control and LP-treated groups, respectively. (B) Distribution of DEGs up-regulated or down-regulated in leaves and roots of QXN233 compared with QXH0121 in the control and LP-treated groups, respectively.
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