Nakahama N, Ito A, Kaneko S, Matsuki Y, Suyama Y, Hayano A, Murayama M, Isagi Y.
Genes Genet Syst. 2018 Jan 17. doi: 10.1266/ggs.17-00021
Abstract
Microsatellite markers were developed for the endangered orchid Calanthe izu-insularis (Orchidaceae). This species is unique to the Izu Islands in Japan. Unfortunately, its population size has decreased because of excessive collection for horticultural purposes. In addition, although natural hybridization between C. izu-insularis and C. discolor var. discolor has been reported, morphological differences between C. izu-insularis and the hybridized individuals remain unclear. Using next-generation sequencing, 11 polymorphic microsatellite markers were developed. All developed markers could amplify C. aristulifera and nine markers could amplify C. d. var. discolor, two other orchid species that are also endangered in Japan. The number of alleles and expected heterozygosity at each locus were 1-6 (mean, 2.35) and 0.00-0.79 (mean, 0.30), respectively. These microsatellite markers will help conservation geneticists in their investigation of the proportion of pure C. izu-insularis individuals in the Izu Islands.
See: https://www.ncbi.nlm.nih.gov/pubmed/29343671
Figure 1: Principal coordinates analysis plots of individuals based on co-dominant genotypic distances (Smouse and Peakall 1999). Axis 1 explains 64.49% of the variance and axis 2 explains 5.17% of the variance.
|
[ Other News ]___________________________________________________
|