Das G, Rao GJN, Varier M, Prakash A, Prasad D
Sci Rep. 2018 Feb 5; 8(1):2413. doi: 10.1038/s41598-018-20495-x.
Abstract
Rice, a major food crop, is grown in a wide range of ecological conditions and suffers significant yield losses as it is constantly exposed to a wide range of environmental and biotic stresses. The prevalence of different biotypes/strains has necessitated assembling of numerous resistance genes/QTLs into elite genotypes to confer a broader scale of resistance. The current study reports successful pyramiding of genes/QTLs that confer tolerance/resistance to submergence (Sub1), salinity (Saltol), blast (Pi2, Pi9) and gall midge (Gm1, Gm4) to supplement the four bacterial blight resistance genes (Xa 4, xa5, xa13, Xa21) present in improved Tapaswini, an elite cultivar. The precise transfer of genes/QTLs was accomplished through effective foreground selection and suitable gene pyramids were identified. Background selection was practiced using morphological and grain quality traits to enhance the recovery of the recurrent parental genome. In the bioassays, the pyramids exhibited higher levels of resistance/ tolerance against the target stresses. The novel feature of the study was successful pyramidization and demonstration of the function of ten genes/QTLs in a new genotype. This success can stimulate several such studies to realize the full potential of molecular plant breeding as the foundation for rice improvement.
See https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5799378/
Figure 1: Parental polymorphism survey through PCR assays (cropped gels displayed). (A) Amplification with RG64 (digested with HaeIII) linked to Pi2. (B) Amplification with P28 linked to Pi9. (C) Amplification with RM444 linked to Gm1. (D) Amplification with RM547 linked to Gm4. (E) Amplification with SUB1BC2 linked to Sub1. (F) Amplification with RM10745 linked to Saltol. Arrows indicates amplicons linked to resistance and susceptible alleles.
|
[ Other News ]___________________________________________________
|