Yueling Li, Dandan Li, Zongli Guo, Qiangsheng Shi, Shuangxi Xiong, Cheng Zhang, Jun Zhu and Zhongnan Yang
Abstract
Background
Sporopollenin is a major component of the pollen exine pattern. In Arabidopsis, acyl-CoA synthetase5 (ACOS5) is involved in sporopollenin precursor biosynthesis. In this study, we identified its orthologue, OsACOS12, in rice (Oryza sativa) and compared the functional conservation of ACOS in rice to Arabidopsis.
Results
Sequence analysis showed that OsACOS12 shares 63.9 % amino acid sequence identity with ACOS5. The osacos12 mutation caused by a pre-mature stop codon in LOC_Os04g24530 exhibits defective sexine resulting in a male sterile phenotype in rice. In situ hybridization shows that OsACOS12 is expressed in tapetal cells and microspores at the transcript level. The localization of OsACOS12-GFP demonstrated that OsACOS12 protein is accumulated in tapetal cells and anther locules. OsACOS12 driven by the ACOS5 promoter could partially restore the male fertility of the acos5 mutant in Arabidopsis.
Conclusions
OsACOS12 is an orthologue of ACOS5 that is essential for sporopollenin synthesis in rice. ACOS5 and OsACOS12 are conserved for pollen wall formation in monocot and dicot species.
See: http://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-016-0943-9
BMC Plant Biology, 21 November 2016;
Fig. 1
OsACOS12 in O. sativa is an orthologue of ACOS5 in Arabidopsis. a Amino acid sequences alignment of OsACOS12 and ACOS5. The sequences were aligned using Clustal W and displayed using BOXSHADE. b A neighbour-joining phylogenetic tree of OsACOS12 and its orthologues in different species. Bootstrap values are the percentage of 1,000 replicates. The conserved AMP binding domain and fatty acid binding domain are indicated.
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