Phenotypic Characterization, Fine Mapping, and Altered Expression Profiling of Roses1 Mutation That Affects Organ Size and Water Loss Through Regulating Stomatal Density in Rice
Tuesday, 2018/03/27 | 08:11:52
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Crop Science; Received: Oct 27, 2016; Accepted: Aug 22, 2017; Published: January 12, 2018 Vol. 58 No. 2, p. 486-506 AbstractOrgan size is an important agronomic trait that directly affects the biomass of rice (Oryza sativa L.), thus identification and characterization of genes involved in organ size control would contribute to basic biology, as well as provide target genes for genetic manipulation of rice yield potential. Although organism or organ size is of pivotal importance, the molecular and genetic mechanisms underlying it remain far from understood. Here we report the isolation and characterization of reduced organ size with early senescence1 (roses1) mutant in rice. The roses1 mutant was obtained by ethyl methanesulfonate mutagenesis, and genetic analysis revealed that roses1 mutation is controlled by a single recessive nuclear gene. Distinct reduction in the size of organs in roses1 mutant plants was attributed to decreased cell number and cell size detected by histological analysis and the early leaf senescence with green and pale brown stripes, probably due to elevated stomata density detected by microscopy analysis. The ROSES1 gene was isolated by using map-based cloning strategy, encoding a BEL1-like homeobox transcription factor containing a plant-specific peroxidase (POX) domain of unknown function. The β-glucuronidase (GUS) activity driven by the ROSES1 promoter was strongly detected at the root meristem and elongation zone, shoot meristem, node, intercalary meristem of internode, leaf, inflorescence branch, and developing caryopsis and embryo. Differential gene expression analysis revealed potential regulatory networks involved in organ size control and stomata functioning that could be affected by the expression of the ROSES1 protein.
See: https://dl.sciencesocieties.org/publications/cs/abstracts/58/2/486
Fig. 1. Phenotypic characterization of the roses1 mutant and its wild-type (WT) Fuhui9802 plant. (a) 10-d-old plants, bar = 1 cm. (b) 60-d-old plants, bar = 5 cm. (c) Mature plants, bar = 10 cm. (d) The separated internodes and the panicles of WT and roses1, bar = 10 cm. (e) Transverse section of the middle part of the second internode from the top in the WT and roses1, bar = 0.2 cm. (f) Longitudinal sections of culms, bars = 20 μm. (g, h) Cross-sections of culms of WT and roses1 plants, bars = 50 μm. (i) Length comparison of first to third leaves between the WT and roses1 mutant at heading stage, bars = 10 cm. (j, k) Transverse sections of the 12th leaf blade of WT and roses1 plants, bars = 2 mm. (l) Spikelets of WT and roses1, bars = 2 mm. (m) Flowers of WT and roses1, bars = 2 mm. (n) Pollen grains sampled from spikelets just before flowering and stained with iodine and potassium iodide solution, bars = 0.3 mm. (o) Panicles of the WT and roses1 plants, bar = 1 cm. (p, q) Comparison of length and width of grains and seeds between the WT and roses1 mutant, bar = 0.5 cm. (r ) Comparison of the unhusked grains and the separated embryos between the WT and roses1 mutant, bar = 0.5 cm. |
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