Chu S, Li H, Zhang X, Yu K, Chao M, Han S, Zhang D.
Int J Mol Sci. 2018 Jun 6;19(6). pii: E1688. doi: 10.3390/ijms19061688.
Abstract
Previous studies have revealed a significant genetic relationship between phosphorus (P)-efficiency and photosynthesis-related traits in soybean. In this study, we used proteome profiling in combination with expression analysis, biochemical investigations, and leaf ultrastructural analysis to identify the underlying physiological and molecular responses. The expression analysis and ultrastructural analysis showed that the photosynthesis key genes were decreased at transcript levels and the leaf mesophyll and chloroplast were severely damaged after low-P stress. Approximately 55 protein spots showed changes under low-P condition by mass spectrometry, of which 17 were involved in various photosynthetic processes. Further analysis revealed the depression of photosynthesis caused by low-P stress mainly involves the regulation of leaf structure, adenosine triphosphate (ATP) synthesis, absorption and transportation of CO₂, photosynthetic electron transport, production of assimilatory power, and levels of enzymes related to the Calvin cycle. In summary, our findings indicated that the existence of a stringent relationship between P supply and the genomic control of photosynthesis in soybean. As an important strategy to protect soybean photosynthesis, P could maintain the stability of cell structure, up-regulate the enzymes’ activities, recover the process of photosystem II (PSII), and induce the expression of low-P responsive genes and proteins.
See https://www.ncbi.nlm.nih.gov/pubmed/29882786
Figure 1: Effects of low-P stress on morphology and photosynthetic parameters of soybean seedling. Five days old germinated plants were transferred on Hogland nutrient solution (Optimum P, +P, 1000 μmol KH2PO4; Low-P, −P, 5 μmol KH2PO4) and allowed to grow for two weeks under hydroponic conditions. (a) Soybean grown under optimum P conditions (left) and −P conditions (right); (b) The effects of low-P stress on soybean photosynthetic parameters chlorophyll content (CC), net photosynthetic rate (Pn), maximum quantum efficiency of PSII (Fv/Fm) and quantum efficiency of PSII (ΦPSII). ANOVA was used to compare significance and the lowercase/capital letter denote significantly different at p < 0.05/0.01 (Least Significant Difference, LSD test).
|
[ Other News ]___________________________________________________
|