Jidong Cao, Chao Yang, Lingjuan Li, Lan Jiang, Yao Wu, Chuanwan Wu, Qingyun bu, Guixian Xia, Xiaoyun Liu, Yuanming Luo and Jun Liu
Mol Plant Microbe Interact. 2016 Nov 1. [Epub ahead of print]
ABSTRACT
Plant plasma membrane (PM) plays important roles in immune response. Here, we utilized quantitative mass spectrometry to explore the rice PM protein composition 34 and dynamic changes during Magnaporthe oryzae (M. oryzae) infection. We report thus far the largest rice PM Proteome dataset with 3,906 identified proteins, among which 484 proteins were differentially expressed after M. oryzae infection. One third of the identified proteins are predicted to have at least one transmembrane domain. Half of the identified proteins are predicted to have binding functions and over one third of the proteins have enzyme?related functions. In addition, GO analyses revealed that abscisic acid (ABA) and cytokinin (CK) signaling were sequentially activated after M. oryzae infection in rice. We found that the activation of ABA signaling and the suppression of rice immune response occurred at early infection stage, while the activation of CK signaling, the upregulation of sugar transporter genes expression and the nutrient efflux of infected rice cells occurred at later infection stage. Thus, we further propose that M. oryzae activates ABA signaling to repress rice immune signaling for initial invasion and redirects nutrient efflux of infected cells for massive growth at later infection stage.
See https://www.ncbi.nlm.nih.gov/pubmed/27800704
Fig. 2. GO biological process of differentially expressed proteins (DEPs). A, DEPs at different time points. The pathogen and mock-treated rice leaves were used for PM protein enrichment. The detected proteins compared to their respective datasets with the threshold of Log2 fold change ≥0.5 or ≤-0.5 were considered as DEPs. The number of DEPs are displayed by 24 and 48 hpi, respectively. B, Classification of the DEPs into molecular functions and biological processes. The homology-based annotation was performed by Blast2Go software to analyze the DEPs. The red slices represent the upregulated proteins, and grey slices represent downregulated proteins. C, Heat map representing the differentially regulated immune proteins. The DEPs were subjected to homology-based annotation by Blast2GO against RGAP database.
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