ThPP1 gene, encodes an inorganic pyrophosphatase in Thellungiella halophila, enhanced the tolerance of the transgenic rice to alkali stress
Tuesday, 2017/12/19 | 08:13:25
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ThPP1 gene, encodes an inorganic pyrophosphatase in Thellungiella halophila, enhanced the tolerance of the transgenic rice to alkali stress Rui He, Guohong Yu, Xiaori Han, Jiao Han, Wei Li, Bing Wang, Shengcai Huang, Xianguo Cheng Plant Cell Reports December 2017, Volume 36, Issue 12, pp 1929–1942 AbstractKey messageAn inorganic pyrophosphorylase gene, ThPP1, modulated the accumulations of phosphate and osmolytes by up-regulating the differentially expression genes, thus enhancing the tolerance of the transgenic rice to alkali stress (AS). AbstractInorganic pyrophosphorylase is essential in catalyzing the hydrolysis of pyrophosphate to inorganic phosphate during plant growth. Here, we report the changes of physiological osmolytes and differentially expression genes in the transgenic rice overexpressing a soluble inorganic pyrophosphatase gene ThPP1 of Thellungiella halophila in response to AS. Analyses showed that the ThPP1 gene was a PPase family I member which is located to the cytoplasm. Data showed that the transgenic lines revealed an enhanced tolerance to AS compared to the wild type, and effectively increased the accumulations of inorganic phosphate and organic small molecules starch, sucrose, proline and chlorophyll, and maintained the balance of osmotic potential by modulating the ratio of Na+/K+ in plant cells. Under AS, total 379 of differentially expression genes were up-regulated in the leaves of the transgenic line compared with control, and the enhanced tolerance of the transgenic rice to the AS seemed to be associated with the up-regulations of the osmotic stress-related genes such as the L-type lectin-domain containing receptor kinase (L-type LecRK), the cation/H+ antiporter gene and the vacuolar cation/proton exchanger 1 gene (CAX1), which conferred the involvements in the biosynthesis and metabolic pathways. Protein interaction showed that the ThPP1 protein specifically interacted with a 16# target partner of the photosystem II light-harvesting-Chl-binding protein. This study suggested that the ThPP1 gene plays an important regulatory role in conferring the tolerance of the transgenic rice to AS, and is an effective candidate in molecular breeding for crop cultivation of the alkali tolerance.
See: https://link.springer.com/article/10.1007/s00299-017-2208-y
Figure 1: Bioinformatics analysis of the ThPP1 gene. a Phylogenetic tree analysis. Green color represents dicotyledon; yellow color represents arbor. b Homology alignments inorganic pyrophosphorylase. ThPP1, Thellungiella halophila (KC250018.1); AtPP1, Arabidopsis thaliana (NP_196527.1); PtPP1, Populus trichocarpa (XP_002298803.1); GmPP1, Glycine max (XP_003543117.1); ZmPP1, Zea mays (NP_001140851.1). Blue color indicates the similarity; white color indicates the differences. c The predicted three division structure of the ThPP1 protein (color figure online) |
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