Shi WY, Du YT, Ma J, Min DH, Jin LG, Chen J, Chen M, Zhou YB, Ma YZ, Xu ZS, Zhang XH.
Int J Mol Sci. 2018 Dec 17;19(12). pii: E4087. doi: 10.3390/ijms19124087.
Abstract
WRKYs are important regulators in plant development and stress responses. However, knowledge of this superfamily in soybean is limited. In this study, we characterized the drought- and salt-induced gene GmWRKY12 based on RNA-Seq and qRT-PCR. GmWRKY12, which is 714 bp in length, encoded 237 amino acids and grouped into WRKY II. The promoter region of GmWRKY12 included ABER4, MYB, MYC, GT-1, W-box and DPBF cis-elements, which possibly participate in abscisic acid (ABA), drought and salt stress responses. GmWRKY12 was minimally expressed in different tissues under normal conditions but highly expressed under drought and salt treatments. As a nucleus protein, GmWRKY12 was responsive to drought, salt, ABA and salicylic acid (SA) stresses. Using a transgenic hairy root assay, we further characterized the roles of GmWRKY12 in abiotic stress tolerance. Compared with control (Williams 82), overexpression of GmWRKY12enhanced drought and salt tolerance, increased proline (Pro) content and decreased malondialdehyde (MDA) content under drought and salt treatment in transgenic soybean seedlings. These results may provide a basis to understand the functions of GmWRKY12 in abiotic stress responses in soybean.
See: https://www.ncbi.nlm.nih.gov/pubmed/30562982
Figure 1. Expression pattern of fifty-three GmWRKYs in six different tissues (young leaf, flower, pod shell, seed, root and nodule). The fifty-three GmWRKYs were selected from drought treatment RNA-Seq data based on the rule that log2(GH_treat/CK1_treat) >1. The tissue expression is from SoyDB (http://soykb.org/). The color legend refers to the different expression level under normal condition. “DAF” in the tissue label indicates days after flowering.
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