Zulfiqar Ali Sahito, Lixiang Wang, Zhengxi Sun, Qiqi Yan, Xingke Zhang, Qiong Jiang, Ihteram Ullah, Yiping Tong and Xia Li
BMC Plant Biology; 1 December 2017; 17:229
Abstract
Background
Plant roots are highly plastic to high salinity. However, the molecular mechanism by which root developmental plasticity is regulated remains largely unknown. Previously we reported that miR172c-NNC1 module plays a key role in soybean-rhizobial symbiosis. The fact that the miR172c promoter contains several stress-related cis elements indicates that miR172c may have a role in root response to abiotic stress.
Results
Here we showed that miR172c is greatly induced by salt stress in soybean. Overexpression of miR172c and knockdown of miR172c activity resulted in substantially increased and reduced root sensitivity to salt stress, respectively. Furthermore, we show that the target gene NNC1 (Nodule Number Control 1) of miR172c was downregulated by salt stress. The transgenic roots overexpressing or knocking down NNC1 expression also exhibited the altered root sensitivity to salt stress.
Conclusion
The study reveals the crucial role of miR172c-NNC1 module in root stress tolerance to salt stress in soybean.
See: https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-017-1161-9
Figure 1: Analysis of the miR172c promoter and miR172c expression. a Computational analysis of the regulatory cis elements of the miR172c promoter. The promoter sequence (2000 bp) of upstream of pre-miR172c (www.phytozome.net/) was chosen for multiple cis-element analysis using an online software at http://www.dna.affrc.go.jp/place/. b qRT-PCR analysis of expression of miR172c in roots. Seven day-old seedlings were treated with 75 mM NaCl, and roots were harvested at 0, 1, 3, 6, 12, and 24 h after treatment. miR1515a was used to normalize transcript abundance. Data are mean ± SD from three biological repeats. Letters indicate significant differences from the empty vector controls according to the Student’s Newman-Kuels test (P < 0.05). c Histochemical analysis of the expression of miR172c. GUS staining was performed using the transgenic roots expression promiR172c::GUS treated with or without 75 mM NaCl for 24 h. Two weeks-old composite plants were used to conduct the experiments. Bars in (c) =1 cm.
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