Sun D, Cao H, Shi Y, Huang P, Dong B, Liu X, Lin F, Lu J.
Mol Plant Pathol. 2016 Jul 19. doi: 10.1111/mpp.12461. [Epub ahead of print]
Abstract
Magnaporthe oryzae is a cereal pathogen causing 20%-30% rice yield losses. Regulatory Factor X transcription factors are highly conserved proteins with diverse functions among organisms. Here, we show that MoRfx1 is required for cell division, development, and pathogenicity in M. oryzae. Deletion of MoRFX1 resulted in reduced growth and conidiation, lowered appressorium turgor, and impaired virulence. ΔMorfx1 displayed increased sensitivity to UV light, 4 DNA-damaging agents, and 3 cell wall perturbing compounds. However, ΔMorfx1 showed decreased sensitivity to bleomycin, a DNA/cell-wall-damaging agent, and increased chitin content of cell wall in vegetative mycelium. Additionally, cell division speed was reduced in ΔMorfx1, and ΔMorfx1 did not produce three-celled conidia. RNA-seq and qPCR analyses suggested that MoRfx1 has bipartite functions in controlling the expression of genes required for cell division and chitin metabolism, not only as a transcriptional repressor, but also as a transcriptional activator. Particularly, the expression of chitin deacetylase genes MoCDA2 and MoCDA1 was greatly down-regulated in ΔMorfx1; and deletion of MoCDA2 and MoCDA1, similar to ΔMorfx1, increased resistance to bleomycin. Taken together, our results indicate that MoRFX1 regulates development and pathogenicity by modulating the expression of genes involved in cell division and cell wall integrity. This article is protected by copyright. All rights reserved.
See http://www.ncbi.nlm.nih.gov/pubmed/27434465
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