Yang R, Li J, Zhang H, Yang F, Wu Z, Zhuo X, An X, Cheng Z, Zeng Q, Luo Q.
Plant Genome. 2018 Nov;11(3). doi: 10.3835/plantgenome2017.11.0097.
Abstract
Nees & Arn. ex Watt, a perennial wild rice species with a GG genome, preserves many important genes for cultivated rice ( L.) improvement. At present, however, no genetic resource is available for studying . Here, we report 91,562 high-quality transcripts of assembled de novo. Moreover, comparative transcriptome analysis revealed that 1311 single-copy orthologous pairs shared by and (Zoll. & Moritzi) Baill. that may have undergone adaptive evolution. We performed an analysis of the genes potentially involved in plant-pathogen interactions to explore the molecular basis of disease resistance, and isolated the full-length cDNAs of () and () orthologs from . The overexpression of in Nipponbare and functional characterization showed enhanced the resistance of transgenic Nipponbare to rice blast resulting from the presence of the gene. , an alternatively spliced transcript of the blast resistance gene in encodes a 1024-amino acid polypeptide with a C-terminal thioredoxin domain. This study provides an important resource for functional and evolutionary studies of the genus.
See: https://www.ncbi.nlm.nih.gov/pubmed/30512031
Fig. 1. The annotation and functional classification of assembled unigenes. (A) Venn diagram of the annotation results of unigenes searched against four protein databases. (B) Gene Ontology (GO) classifications. (C) Histogram of Cluster of Orthologous Groups (COG) classifications. (D) The top 20 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway terms. Classifications are based on the categories: metabolism (red), genetic information processing (orange), environmental information processing (green), cellular processes (blue), and organismal systems (purple).
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