NURHAYATI, SINTHO WAHYUNING ARDIE, TRI JOKO SANTOSO, SUDARSONO
B I O D I V E R S I T A S Volume 22, Number 9, September2021 Pages: 3792-3800
Abstract
Nurhayati, Ardie SW, Santoso TJ,Sudarsono. 2021. CRISPR/Cas9-mediated genome editing in rice cv. IPB3Sresults in a semi-dwarf phenotypic mutant.Biodiversitas 22:3792-3800.IPB3Sis Indonesian lowland rice and high-yielding cultivar. However, plant height posture makes itprone tolodging whichcould reduce the yield. This study aimed to edit the GA20Ox2gene by introducing CRISPR/Cas9 GA20Ox2construct into IPB3Sand developing the semidwarf rice mutants. Immature embryo explants of IPB3Swere used for thetransformation process mediated by EHA105 strain of Agrobacterium tumefacienscarrying pC1300-Cas9/GA20Ox2and by changing the regeneration medium composition. PCR analysis showed that rice cv. IPB3Sgenetic transformation gained putative mutant T0 lines carrying the hptgene (growthefficiency was 47.9%, while transformation efficiency was 19.3%). Using the developed regeneration medium, we have obtained 24putative rice cv. IPB3ST0 mutant lines carrying hpt. The best medium for regenerating IPB3S was the Amedium (regeneration efficiency 73.3%). IPB 8 and IPB 14 were potential to evaluate in the next generation. The shortest plant height for the T1 generation was observed inthe IPB 8-3mutant.
See: https://www.smujo.id/biodiv/article/view/9132/5114
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