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Recombinant transfer in the basic genome of Escherichia coli
Saturday, 2015/07/25 | 06:12:41
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Purushottam D. Dixit, Tin Yau Pang, F. William Studier, and Sergei Maslov EVOLUTION SignificanceA significant fraction of the length of Escherichia coli genomes comprises mobile elements integrated at various sites in a ∼4-Mbp basic genome shared by the species. We find that the entire basic genome is continually exchanged by homologous recombination with genome fragments acquired from other genomes in the population. Evolutionary groups appear to exchange DNA preferentially within the same group but also with other groups to different extents. Entering DNA is often fragmented by restriction systems of the recipient cell, with surviving pieces replacing homologous parts of the recipient chromosome. Coevolving populations of phages that package genome fragments and deliver them to cells that have appropriate receptors are likely mediators of most DNA transfers, distributing variability throughout the species. AbstractAn approximation to the ∼4-Mbp basic genome shared by 32 strains of Escherichia coli representing six evolutionary groups has been derived and analyzed computationally. A multiple alignment of the 32 complete genome sequences was filtered to remove mobile elements and identify the most reliable ∼90% of the aligned length of each of the resulting 496 basic-genome pairs. Patterns of single base-pair mutations (SNPs) in aligned pairs distinguish clonally inherited regions from regions where either genome has acquired DNA fragments from diverged genomes by homologous recombination since their last common ancestor. Such recombinant transfer is pervasive across the basic genome, mostly between genomes in the same evolutionary group, and generates many unique mosaic patterns. The six least-diverged genome pairs have one or two recombinant transfers of length ∼40–115 kbp (and few if any other transfers), each containing one or more gene clusters known to confer strong selective advantage in some environments. Moderately diverged genome pairs (0.4–1% SNPs) show mosaic patterns of interspersed clonal and recombinant regions of varying lengths throughout the basic genome, whereas more highly diverged pairs within an evolutionary group or pairs between evolutionary groups having >1.3% SNPs have few clonal matches longer than a few kilobase pairs. Many recombinant transfers appear to incorporate fragments of the entering DNA produced by restriction systems of the recipient cell. A simple computational model can closely fit the data. Most recombinant transfers seem likely to be due to generalized transduction by coevolving populations of phages, which could efficiently distribute variability throughout bacterial genomes.
See: http://www.pnas.org/content/112/29/9070.abstract.html?etoc PNAS July 21, 2015 vol. 112 no. 29 9070-9075
Fig. 1. Phylogenetic tree derived from filtered genome-wide average SNP densities (Δ) between 496 pairs of 32 basic genomes. Previously recognized phylogenetic groups: E (light blue), A (green), B1 (blue), D2 (yellow), D1 (brown), and B2 (red). The tree was calculated using UPGMA algorithm. Dots in the lines connecting pairs of evolutionary groups are placed approximately at average SNP densities between them. The groups are ordered to fit the relative divergences among them summarized in Table S1. GenBank accession numbers are given in Table S3. |
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(13).png "Recombinant transfer in the basic genome of Escherichia coli")
