Di Qin, Xiao-Yi Liu, Cristina Miceli, Qi Zhang & Pi-wu Wang
BMC Biotechnology volume 19, Article number: 66 (2019); Published: 15 October 2019
Abstract
Background
Cry8-like from Bacillus thuringiensis (Bt) encodes an insecticidal crystal (Cry) protein. Holotrichia parallela (Coleoptera: Scarabaeoidae), commonly known as the dark black chafer, is a troublesome pest of soybean (Glycine max). To test whether cry8-like can confer resistance against H. parallela to soybean, we introduced cry8-like from the Bt strain HBF-18 into soybean cultivar Jinong 28.
Results
Quantitative reverse transcription-PCR analysis demonstrated that cry8-like was expressed most highly in soybean leaves. In addition, Southern blot assays revealed that one copy of the integrated fragment was present in the transformed plants. Eight independent cry8-like transgenic lines were subsequently fed on by H. parallela. Under H. parallela feeding stress, the survival rates of the non-transgenic plants were 92% lower than those of the transgenic plants. The mortality rate of H. parallela increased when the larvae fed on the roots of T1 transgenic soybean plants. Moreover, the surviving larvae were deformed, and their growth was inhibited.
Conclusions
Collectively, our data suggest that transgenic soybean plants expressing the cry8-like gene are more resistant to H. parallela than non-transgenic plants and that transgenic expression of the cry8-like gene may represent a promising strategy for engineering pest tolerance. The events generated in this study could thus be utilized in soybean breeding programs.
See https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-019-0563-1
Figure 2: Agrobacterium-mediated transformation of soybean. (a) Diagram of pCAMBIA3300-cry8-like used for transformation. (b) Soybean cotyledon nodes cultured on shoot-induction medium (c) and (d) Shoot development. (e) 3-month-old T0 transgenic soybean plants grown under greenhouse conditions in soil
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