Liu XH, Ning GA, Huang LY, Zhao YH, Dong B, Lu JP, Lin FC.
Sci Rep. 2016 Aug 9;6:31204. doi: 10.1038/srep31204.
Abstract
Calpains are ubiquitous and well-conserved proteins that belong to the calcium-dependent, non-lysosomal cysteine protease family. In this study, 8 putative calpains were identified using Pfam domain analysis and BlastP searches in M. oryzae. Three single gene deletion mutants (ΔMocapn7, ΔMocapn9 and ΔMocapn14) and two double gene deletion mutants (ΔMocapn4ΔMocapn7 and ΔMocapn9ΔMocapn7) were obtained using the high-throughput gene knockout system. The calpain disruption mutants showed defects in colony characteristics, conidiation, sexual reproduction and cell wall integrity. The mycelia of the ΔMocapn7, ΔMocapn4ΔMocapn7 and ΔMocapn9ΔMocapn7 mutants showed reduced pathogenicity on rice and barley.
See http://www.nature.com/articles/srep31204
Figure 6: (A) Disease symptoms on rice inoculated with conidial suspension from Guy11 and mutants. Typical leaves were photographed 7 days after inoculation. (B) Disease symptoms on the cut leaves of barley and rice inoculated with mycelial plugs from Guy11 and mutants. Typical leaves were photographed 4 days after inoculation. (C) Development of appressorium-like structures and penetration assays on barley with Guy11 and mutants. Scale bar = 10 μm.
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