Zhang J, Yin K, Sun J, Gao J, Du Q, Li H, Qiu JL
Plant Biotechnol J. 2017 Jul 6. doi: 10.1111/pbi.12787. [Epub ahead of print]
Abstract
Direct control of protein level enables rapid and efficient analyses of gene functions in crops. Previously, we developed the RDDK-Shield1 (Shld1) system in the model plant Arabidopsis thaliana for direct modulation of protein stabilization using a synthetic small molecule. However, it was unclear whether this system is applicable to economically important crops. In this study we show that the RDDK-Shld1 system enables rapid and tunable control of protein levels in rice and wheat. Accumulation of RDDK-fusion proteins can be reversibly and spatio-temporally controlled by the synthetic small-molecule Shld1. Moreover, RDDK-Bar and RDDK-Pid3 fusions confer herbicide and rice blast resistance, respectively, in a Shld1-dependent manner. Therefore, the RDDK-Shld1 system provides a reversible and tunable technique for controlling protein functions and conditional expression of transgenes in crops.
See: https://www.ncbi.nlm.nih.gov/pubmed/28682500
Figure 2: Temporal and spatial control of RDDK-EGFP accumulation by Shld1 in rice.
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