Asha S, Soniya EV.
Sci Rep. 2017 Feb 1;7:41052. doi: 10.1038/srep41052.
Abstract
Small RNAs derived from ribosomal RNAs (srRNAs) are rarely explored in the high-throughput data of plant systems. Here, we analyzed srRNAs from the deep-sequenced small RNA libraries of Piper nigrum, a unique magnoliid plant. The 5' end of the putative long form of 5.8S rRNA (5.8SLrRNA) was identified as the site for biogenesis of highly abundant srRNAs that are unique among the Piperaceae family of plants. A subsequent comparative analysis of the ninety-seven sRNAomes of diverse plants successfully uncovered the abundant existence and precise cleavage of unique rRF signature small RNAs upstream of a novel 5' consensus sequence of the 5.8S rRNA. The major cleavage process mapped identically among the different tissues of the same plant. The differential expression and cleavage of 5'5.8S srRNAs in Phytophthora capsici infected P. nigrum tissues indicated the critical biological functions of these srRNAs during stress response. The non-canonical short hairpin precursor structure, the association with Argonaute proteins, and the potential targets of 5'5.8S srRNAs reinforced their regulatory role in the RNAi pathway in plants. In addition, this novel lineage specific small RNAs may have tremendous biological potential in the taxonomic profiling of plants.
See https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5286533/
Figure 2: Distribution and abundance pattern of ribosomal RNA derived small RNAs in Piper nigrum small RNA libraries (A) Distribution of unique srRNA candidates against black pepper rRNA. (B) Abundant srRNA from Pn_CL (upto100 rpm) mapped against rRNA. Most of the abundant reads were mapped at the 5.8S rRNA region. (C) The proportion of 5.8S rRFs in the Pn_CL, Pn_IL and Pn_IR libraries. (D) The length categorisation of 5.8S rRFs among the three libraries.
|
[ Other News ]___________________________________________________
|