C A Sowmiya, J Ramalingam, R Pushpam, D Shoba, K K Kumar, M Arumugam Pillai

Physiol Mol Biol Plants; 2024 Jun; 30(6):1003-1019. doi: 10.1007/s12298-024-01461-6. 

Abstract

Bacterial Leaf Blight (Xanthomonas oryzae pv. oryzae) and blast (Magnaporthe oryzae) are the major biotic stresses around the rice-growing zones of the world. The development of resistant varieties through Marker Assisted Backcross Breeding is the utmost economical and eco-friendly method for achieving stable yield. Amongst the resistance genes recognized, Xa21 and Pi54 possess broad-spectrum resistance to many Xoo and blast strains around the world. In the present study, we have effectively introgressed a Bacterial Blight resistance gene (Xa21) and a blast resistance gene (Pi54) into susceptible variety ADT43 from RP-Bio-Patho-2 coupled with phenotypic selection for agronomic, cooking quality and grain traits through MABC. MABC was sustained till BC₂F₂ generation with specific markers pTA248 for Xa21 and Pi54MAS for Pi54 resistance genes. A set of SSR markers for parental polymorphism were utilized for maximum regaining of recurrent parent genome in each backcrossing. "Positive plants" from BC₂F₁ were selfed to generate BC₂F₂ and the homozygous lines for bacterial leaf blight and blast resistance genes were identified for further assessment.

See https://pubmed.ncbi.nlm.nih.gov/38974353/