Shang S, Wu C, Huang C, Tie W, Yan Y, Ding Z, Xia Z, Wang W, Peng M, Tian L, Hu W.
Genes (Basel). 2018 Feb 20;9(2). pii: E110. doi: 10.3390/genes9020110.
Abstract
GENERAL REGULATORY FACTOR (GRF) proteins play vital roles in the regulation of plant growth, development, and response to abiotic stress. However, little information is known for this gene family in cassava (Manihot esculenta). In this study, 15 MeGRFs were identified from the cassava genome and were clustered into the ε and the non-ε groups according to phylogenetic, conserved motif, and gene structure analyses. Transcriptomic analyses showed eleven MeGRFs with constitutively high expression in stems, leaves, and storage roots of two cassava genotypes. Expression analyses revealed that the majority of GRFs showed transcriptional changes under cold, osmotic, salt, abscisic acid (ABA), and H₂O₂ treatments. Six MeGRFs were found to be commonly upregulated by abiotic stress, ABA, and H₂O₂ treatments, which may be the converging points of multiple signaling pathways. Interaction network analysis identified 18 possible interactors of MeGRFs. Taken together, this study elucidates the transcriptional control of MeGRFs in tissue development and the responses of abiotic stress and related signaling in cassava. Some constitutively expressed, tissue-specific, and abiotic stress-responsive candidate MeGRF genes were identified for the further genetic improvement of crops.
See: https://www.ncbi.nlm.nih.gov/pubmed/29461467
Table S4. Expression data of cassava GRFs in different tissues of W14 and Arg7.
|
Gene
|
Arg7_stem
|
Arg7_leaf
|
Arg7_storage root
|
W14_stem
|
W14_leaf
|
W14_storage root
|
MeGRF1
|
1154,64
|
571,068
|
365,576
|
82,9617
|
133,968
|
147,752
|
MeGRF2
|
0,756377
|
0
|
1,31913
|
4,18406
|
0
|
7,74215
|
MeGRF4
|
234,173
|
37,4154
|
66,1959
|
72,0195
|
42,964
|
125,239
|
MeGRF5
|
331,883
|
32,4061
|
264,738
|
104,304
|
29,116
|
123,229
|
MeGRF6
|
147,812
|
62,0786
|
192,878
|
185,01
|
62,9015
|
218,216
|
MeGRF7
|
302,333
|
68,7126
|
41,8725
|
71,3548
|
31,9812
|
179,232
|
MeGRF8
|
231,288
|
65,1134
|
134,944
|
29,1002
|
29,5099
|
71,8185
|
MeGRF9
|
99,4108
|
3,23397
|
30,7123
|
0,337766
|
0,02948
|
0,0932679
|
MeGRF10
|
141,248
|
95,3195
|
109,238
|
35,9657
|
32,9832
|
60,1744
|
MeGRF11
|
171,927
|
44,2965
|
97,9009
|
79,4895
|
81,4181
|
228,797
|
MeGRF12
|
265,853
|
135,266
|
128,915
|
37,4439
|
49,2718
|
120,315
|
MeGRF13
|
532,464
|
422,694
|
153,389
|
114,001
|
171,484
|
89,4426
|
MeGRF14
|
3,00691
|
0,67314
|
37,1689
|
0,377108
|
2,27194
|
2,46927
|
MeGRF15
|
139,201
|
93,8165
|
51,0452
|
75,3105
|
84,9208
|
146,892
|
|
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