Yunxin Fan, Lu Ma, Xiaoqian Pan, Pujiang Tian, Wei Wang, Kunquan Liu, Ziwei Xiong, Changqing Li, Zhixue Wang, Jianfei Wang, Hongsheng Zhang, Yongmei Bao
Int J Mol Sci.; 2024 Jan 9; 25(2):830. doi: 10.3390/ijms25020830.
Abstract
Rice blast is one of the most devastating diseases, causing a significant reduction in global rice production. Developing and utilizing resistant varieties has proven to be the most efficient and cost-effective approach to control blasts. However, due to environmental pressure and intense pathogenic selection, resistance has rapidly broken down, and more durable resistance genes are being discovered. In this paper, a novel wall-associated kinase (WAK) gene, Pb4, which confers resistance to rice blast, was identified through a genome-wide association study (GWAS) utilizing 249 rice accessions. Pb4 comprises an N-terminal signal peptide, extracellular GUB domain, EGF domain, EGF-Ca2+ domain, and intracellular Ser/Thr protein kinase domain. The extracellular domain (GUB domain, EGF domain, and EGF-Ca2+ domain) of Pb4 can interact with the extracellular domain of CEBiP. Additionally, its expression is induced by chitin and polygalacturonic acid. Furthermore, transgenic plants overexpressing Pb4 enhance resistance to rice blast. In summary, this study identified a novel rice blast-resistant gene, Pb4, and provides a theoretical basis for understanding the role of WAKs in mediating rice resistance against rice blast disease.
See https://pubmed.ncbi.nlm.nih.gov/38255904/
Figure 4. Identification of the rice blast-resistant candidate genes. (A) Local Manhattan plot (top) and LD heatmap (bottom) on BRL10 (left). Candidate genes and significant SNPs in the selected LD block (right). (B–D) qRT-PCR analysis of the candidate genes during M. oryzae infection. The x-axis indicates the time points post-inoculation. The y-axis indicates the relative transcription level of candidate genes. Error bars, mean ± SD (n = 3).
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