Tianzhen Zhang, Yan Hu, Wenkai Jiang, Lei Fang, Xueying Guan, Jiedan Chen, Jinbo Zhang, Christopher A Saski, Brian E Scheffler, David M Stelly, Amanda M Hulse-Kemp, Qun Wan, Bingliang Liu, Chunxiao Liu, Sen Wang, Mengqiao Pan, Yangkun Wang, Dawei Wang, Wenxue Ye, Lijing Chang, Wenpan Zhang, Qingxin Song, Ryan C Kirkbride, Xiaoya Chen, Elizabeth Dennis et al.
Nature Biotechnology 20 April 2015 Published online
http://www.nature.com/nbt/journal/vaop/ncurrent/full/nbt.3207.html#affil-auth
Upland cotton is a model for polyploid crop domestication and transgenic improvement. Here we sequenced the allotetraploid Gossypium hirsutum L. acc. TM-1 genome by integrating whole-genome shotgun reads, bacterial artificial chromosome (BAC)-end sequences and genotype-by-sequencing genetic maps. We assembled and annotated 32,032 A-subgenome genes and 34,402 D-subgenome genes. Structural rearrangements, gene loss, disrupted genes and sequence divergence were more common in the A subgenome than in the D subgenome, suggesting asymmetric evolution. However, no genome-wide expression dominance was found between the subgenomes. Genomic signatures of selection and domestication are associated with positively selected genes (PSGs) for fiber improvement in the A subgenome and for stress tolerance in the D subgenome. This draft genome sequence provides a resource for engineering superior cotton lines.
![Sequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement Domestication of allotetraploid cotton.](/Images_upload/images/image001(75).jpg)
Figure 1 Fiber phenotypes of domesticated allotetraploid cotton (G. hirsutum L. acc. TM-1), wild cotton (G. hirsutum L. acc. yucatanense), and their two closest extant progenitors, G. herbaceum and G. raimondii. Scale bar, 10 mm.
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