Jadwiga Śliwka, Dorota Sołtys-Kalina, Katarzyna Szajko, Iwona Wasilewicz-Flis, Danuta Strzelczyk-Żyta, Ewa Zimnoch-Guzowska, Henryka Jakuczun, Waldemar Marczewski
Theoretical and Applied Genetics; January 2016, Volume 129, Issue 1, pp 131-140
Abstract
Key message
Most QTL for leaf sucrose content map to positions that are similar to positions of QTL for tuber starch content in diploid potato.
Abstract
In the present study, using a diploid potato mapping population and Diversity Array Technology (DArT) markers, we identified twelve quantitative trait loci (QTL) for tuber starch content on seven potato chromosomes: I, II, III, VIII, X, XI, and XII. The most important QTL spanned a wide region of chromosome I (42.0–104.6 cM) with peaks at 63 and 84 cM which explained 17.6 and 19.2 % of the phenotypic variation, respectively. ADP-glucose pyrophosphorylase (AGPase) is the key enzyme for starch biosynthesis. The gene encoding the large subunit of this enzyme, AGPaseS-a, was localized to chromosome I at 102.3 cM and accounted for 15.2 % of the variance in tuber starch content. A more than 100-fold higher expression of this gene was observed in RT-qPCR assay in plants with the marker allele AGPaseS-a1334. This study is the first to report QTL for sucrose content in potato leaves. QTL for sucrose content in leaves were located on eight potato chromosomes: I, II, III, V, VIII, IX, X and XII. In 5-week-old plants, only one QTL for leaf sucrose content was detected after 8 h of darkness; four QTL were detected after 8 h of illumination. In 11-week-old plants, 6 and 3 QTL were identified after dark and light phases, respectively. Of fourteen QTL for leaf sucrose content, eleven mapped to positions that were similar to QTL for tuber starch content. These results provide genetic information for further research examining the relationships between metabolic carbon molecule sources and sinks in potato plants.
See http://link.springer.com/article/10.1007/s00122-015-2615-9/fulltext.html
Fig. 2
QTL on chromosome I for leaf sucrose content detected in population 12-3. QTL were determined by interval mapping and MapQTL® 6 software (Van Ooijen 2009). Sucrose content was measured in four experimental settings: LSC5AN, after 8 h of night in 5-week-old plants (a); LSC5AL, after 8 h of light in 5-week-old plants (b); LSC11AN, after 8 h of night in 11-week-old plants (c); LSC11AL, after 8 h of light in 11-week-old plants (d). Genetic distance (in cM) is shown on the x-axis and QTL effect strength, as indexed by LOD score, is shown on the y-axis. The threshold LOD score (LOD = 3) is indicated by a horizontal line
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