Welcome To Website IAS

Hot news
Achievement

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)

VIFOTEC Award

- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Centres
Website links
Vietnamese calendar
Library
Visitors summary
 Curently online :  6
 Total visitors :  8925093

Extensive changes in gene expression and alternative splicing due to homoeologous exchange in rice segmental allopolyploids
Wednesday, 2019/08/14 | 08:18:43

Zhibin Zhang, Tiansi Fu, Zhijian Liu, Xutong Wang, Hongwei Xun, Guo Li, Baoxu Ding, Yuzhu Dong, Xiuyun Lin, Karen A. Sanguinet, Bao Liu, Ying Wu,  Lei Gong

Theoretical and Applied Genetics; August 2019, Volume 132, Issue 8, pp 2295–2308

Key message

We report rampant homoeologous exchanges in progenies of a newly synthesized rice segmental allotetraploid and demonstrate their consequences to changes of gene expression and alternative splicing.

Abstract

Allopolyploidization is recurrent across the tree of angiosperms and known as a driving evolutionary force in both plants and animals. A salient feature of allopolyploidization is the induction of homoeologous exchange (HE) events between the constituent subgenomes, which may in turn cause changes in gene expression, transcript alternative splicing, and phenotypic novelty. However, this issue has been poorly studied, largely because lack of a system in which the exact parentage donating the subgenomes is known and the HE events are occurring in real time. Here, we employed whole-genome re-sequencing and RNA-seq-based transcriptome profiling in four randomly chosen progeny individuals (at the 10th-selfed generation) of segmental allotetraploids that were constructed by colchicine-mediated whole-genome doubling of F1 hybrids between the two subspecies (japonica and indica) of Asian cultivated Oryza sativa. We show that rampant HE events occurred in these tetraploid individuals, which converted most of the otherwise heterozygous genomic regions into a homogenized state of one parental subgenome. We demonstrate that genes within these homogenized genomic regions in the tetraploids showed high frequencies of altered expression and enhanced alternative splicing relative to their counterparts in the corresponding diploid parents in the embryo tissue. Intriguingly, limited overlaps between the differentially expressed genes and the differential alternative spliced genes were identified, which were partitioned to distinctly enriched gene ontology terms. Together, our results indicate that HE is a major mechanism to rapidly generate novelty in gene expression and transcriptome diversity, which may facilitate phenotypic innovation in nascent allopolyploids and relevant to allopolyploid crop breeding.

 

See https://link.springer.com/article/10.1007/s00122-019-03355-8

Back      Print      View: 421

[ Other News ]___________________________________________________
  • Host plant resistance for fall armyworm management in maize: relevance, status and prospects in Africa and Asia
  • Increasing plant group productivity through latent genetic variation for cooperation
  • THP9 enhances seed protein content and nitrogen-use efficiency in maize
  • The role of soybean 14-3-3 gene (Glyma05g29080) on white mold resistance and nodulation investigations using CRISPR-Cas9 editing and RNA silencing
  • Progress in Soybean Genetic Transformation Over the Last Decade
  • Climate change challenges plant breeding
  • Breeding for disease resistance in soybean: a global perspective
  • The phosphorylation of AMPKβ1 is critical for increasing autophagy and maintaining mitochondrial homeostasis in response to fatty acids
  • Genomic selection for spot blotch in bread wheat breeding panels, full-sibs and half-sibs and index-based selection for spot blotch, heading and plant height
  • Response of Southeast Asian rice root architecture and anatomy phenotypes to drought stress
  • Root Pulling Force Across Drought in Maize Reveals Genotype by Environment Interactions and Candidate Genes
  • Root hair-specific transcriptome reveals response to low phosphorus in Cicer arietinum
  • Protocol for targeted modification of the rice genome using base editing
  • Understanding the Dynamics of Blast Resistance in Rice- Magnaporthe oryzae Interactions
  • Multi-omics analysis reveals the mechanism of seed coat color formation in Brassica rapa L.
  • Highly efficient transgene-free genome editing in tobacco using an optimized CRISPR/Cas9 system, pOREU3TR
  • Breeding of Rc Function Restoration Red Rice via CRISPR/Cas9 Mediated Genome Editing
  • Transposon insertions within alleles of BnaFT.A2 are associated with seasonal crop type in rapeseed
  • Natural allelic variation of GmST05 controlling seed size and quality in soybean
  • Cassava mosaic disease and its management in Southeast Asia

 

Designed & Powered by WEBSO CO.,LTD