Welcome To Website IAS

Hot news
Achievement

Independence Award

- First Rank - Second Rank - Third Rank

Labour Award

- First Rank - Second Rank -Third Rank

National Award

 - Study on food stuff for animal(2005)

 - Study on rice breeding for export and domestic consumption(2005)

VIFOTEC Award

- Hybrid Maize by Single Cross V2002 (2003)

- Tomato Grafting to Manage Ralstonia Disease(2005)

- Cassava variety KM140(2010)

Centres
Website links
Vietnamese calendar
Library
Visitors summary
 Curently online :  10
 Total visitors :  8923507

Optimization of Bacterial Concentration by Filtration for Rapid Detection of Foodborne Escherichia coli O157:H7 Using Real-Time PCR Without Microbial Culture Enrichment.
Friday, 2019/10/18 | 07:21:46

Kim JHOh SW.

J Food Sci. 2019 Oct 11. doi: 10.1111/1750-3841.14836.

Abstract

Escherichia coli O157:H7 is an important foodborne pathogen and has been implicated in numerous food poisoning outbreaks worldwide. Although several microbiological and molecular methods have been developed to detect E. coli O157:H7, the difficulty to rapidly detect low levels of the foodborne bacteria persists. Here, the optimization of a filtration technique to concentrate and rapidly detect E. coli O157:H7 was conducted. Using homogenates prepared from freshly cut lettuce and cabbage samples, the E. coli O157:H7 concentration efficiencies of seven membrane filters were compared. Mixed cellulose ester (MCE) and polyvinylidene difluoride (PVDF) filters demonstrated the highest bacterial recoveries. In addition, the optimal E. coli O157:H7 detachment method from MCE filters after filtration was investigated. Tapping for 80 s was demonstrated to be the most effective method for detaching bacteria from the filters. Further, the possibility of the rapid detection of low levels of E. coli O157:H7 in lettuce and cabbage was evaluated using real-time polymerase chain reaction after bacterial concentration using MCE and PVDF filters. The use of MCE filters enabled the detection of 10° CFU/g (5 CFU/g) of E. coli O157:H7 within 2 hr without microbial enrichment culture. Therefore, concentration by filtration can be used for the rapid detection of low levels of foodborne pathogens. PRACTICAL APPLICATION: The modified method, which has been verified in this study, has been optimized to reduce the analysis time and to detect very low concentrations of E. coli O157:H7 within 2 hr. All these detection systems have a direct economic impact on the food analysis of producers, health authorities, or third-party laboratories.

 

See https://www.ncbi.nlm.nih.gov/pubmed/31604365

Back      Print      View: 350

[ Other News ]___________________________________________________
  • Host plant resistance for fall armyworm management in maize: relevance, status and prospects in Africa and Asia
  • Increasing plant group productivity through latent genetic variation for cooperation
  • THP9 enhances seed protein content and nitrogen-use efficiency in maize
  • The role of soybean 14-3-3 gene (Glyma05g29080) on white mold resistance and nodulation investigations using CRISPR-Cas9 editing and RNA silencing
  • Progress in Soybean Genetic Transformation Over the Last Decade
  • Climate change challenges plant breeding
  • Breeding for disease resistance in soybean: a global perspective
  • The phosphorylation of AMPKβ1 is critical for increasing autophagy and maintaining mitochondrial homeostasis in response to fatty acids
  • Genomic selection for spot blotch in bread wheat breeding panels, full-sibs and half-sibs and index-based selection for spot blotch, heading and plant height
  • Response of Southeast Asian rice root architecture and anatomy phenotypes to drought stress
  • Root Pulling Force Across Drought in Maize Reveals Genotype by Environment Interactions and Candidate Genes
  • Root hair-specific transcriptome reveals response to low phosphorus in Cicer arietinum
  • Protocol for targeted modification of the rice genome using base editing
  • Understanding the Dynamics of Blast Resistance in Rice- Magnaporthe oryzae Interactions
  • Multi-omics analysis reveals the mechanism of seed coat color formation in Brassica rapa L.
  • Highly efficient transgene-free genome editing in tobacco using an optimized CRISPR/Cas9 system, pOREU3TR
  • Breeding of Rc Function Restoration Red Rice via CRISPR/Cas9 Mediated Genome Editing
  • Transposon insertions within alleles of BnaFT.A2 are associated with seasonal crop type in rapeseed
  • Natural allelic variation of GmST05 controlling seed size and quality in soybean
  • Cassava mosaic disease and its management in Southeast Asia

 

Designed & Powered by WEBSO CO.,LTD